Interaction of different yeast species allows bio-economic detection of pharmaceutical residues
Jan 27, 2021
Scientists from the Faculty of Biology at TU Dresden and the Kurt Schwabe Institut für Mess- und Sensortechnik Meinsberg e.V. want to develop an innovativel biological yeast cell-based sensor for the detection of pharmaceutical residues in soil and wastewater in their joint project "Implementation of a yeast pheromone-based signal amplifier system for environmental monitoring of pharmaceutical residues in water" (acronym: ISAr). The project is funded by the Free State of Saxony and the European funding project EFRE.
The active ingredient diclofenac is one of the most widely used anti-inflammatory pharamaceuticals in the world. When consumed orally, 60-70% of the active ingredient is excreted in the urine. Diclofenac thus enters wastewater and, even at low concentrations, can have a negative impact on the environment, for example causing damage to the gills and kidneys of fish.
In the cooperative project ISAr the scientists of the Biological Sensor-Actor Systems group of TU Dresden work together with the Kurt Schwabe Institut für Mess- und Sensortechnik Meinsberg e.V. (KSI Meinsberg e.V.) in order to develop a sustainable and low budget yeast cell-based sensor for the detection of diclofenac in environmentally relevant concentrations in soil and wastewater.
The detector is based on immobilized yeast cells in a suitable technical readout device and is intended to be used as a rapid, on-site alternative to the existing rather expensive laboratory diagnostic methods.
The system is based on yeast cells (Saccharomyces cerevisiae, S.c.) that form a fluorescent protein in the presence of diclofenac. For reliable technical readout, however, the corresponding fluorescence signal must be amplified. For this purpose, the TU Dresden team led by Dr. Kai Ostermann is working on the implementation of an innovative, intrinsic, cell-based amplification system to achieve a significant amplification of the fluorescence signal.
"With the measurement setup developed in the previous BioSAM project, we can detect diclofenac in a range of 5 - 50 μM. However, the sensitivity of this system is still too low to detect the relevant concentrations of diclofenac in wastewater or soil water. Therefore, we aim to increase the sensitivity of diclofenac detection using reporter yeasts to lower the concentration range. To this end, we will first conduct extensive studies on the modulation of cell-cell communication and signal amplification using the yeast pheromone-based system of controlled cell-cell communication that we have developed and patented for the first time. If a reporter yeast reliably communicates that it has detected diclofenac to many other yeast cells and the other yeast cells are thereby stimulated to fluoresce, we can achieve stable signal amplification," Ostermann explains.
Prof. Michael Mertig and his team at KSI Meinsberg will subsequently implement the results achieved in this first process in a real demonstrator setup suitable for on-site measurements using sensor technology. "By increasing the detection sensitivity, such a detector could be used advantageously in various places, including monitoring wastewater from hospitals and retirement homes, the pharmaceutical industry, and in regional wastewater treatment plants," says Prof. Mertig, describing the goal of the project.
More News and Articles
Mar 02, 2021
What do changing routines mean for supply security?
Mar 01, 2021
An open data approach can help water utilities drive efficiencies across their entire operations, says Matthew Hawkridge, chief technology officer, Ovarro
Feb 26, 2021
Are you ready to address the inflow & infiltration entering your collection system, but in need of some guidance? Our new I&I Mitigation Checklist includes steps to take to better understand and combat the costly issue.
Feb 25, 2021
Wastewater treatment technology selected for use on a holiday park because of its ability to withstand seasonal variations in flow, has proven its flexibility during the unexpected events of 2020. The WPL packaged treatment plant was installed 15 the picturesque …
Feb 24, 2021
New Jersey American Water announced today that its employees raised over $26,000 for eight United Way chapters through its annual fundraising campaign. The company also provided an additional donation of $15,000, totaling over $41,000 to help individuals and families in …
Feb 23, 2021
An innovative project delivery approach by the city of Brandenburg in Meade County was the driving force behind a groundbreaking February 5, 2021 for an $8.3 million state-of-the-art wastewater treatment plant.
Feb 22, 2021
TEPPFA, the leading voice of European manufacturers of plastic piping systems welcomes TeraPlast’s decision to renew its membership.
Feb 19, 2021
Olea Edge Analytics, an intelligent edge computing platform for the water utility industry, announced an expanded $3.9 million partnership with the City of Atlanta - Department of Watershed Management that is expected to recover millions of dollars in revenue for …
Feb 18, 2021
The 2021 Risk Management in Underground Construction Course will take place virtually on April 13-15. Registration is open and the tentative agenda has been released.
Feb 17, 2021
British Water has become the latest organisation to pledge its support to the Change the Race Ratio campaign to accelerate racial diversity in business.
Feb 16, 2021
Anue Water Technologies, the manufacturer of cost-effective FORSe and Phantom Oxygen/Ozone injection systems, Enviroprep well-washers, and provider of Anue Geomembrane Covers with embedded carbon-filters and other sustainable and high-performance technologies to prevent odor, corrosion, scale, bacteria, and FOG in municipal …
Feb 15, 2021
Wastewater plants employ a variety of effective and established processes to treat sewage and wastewater. As yet, however, there is no ideal, uniformly recognized method for removing trace substances. Researchers from the Fraunhofer Institute for Environmental, Safety and Energy Technology …